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Updated Single-Molecule Super-Resolution Imaging

New Light Sheet Fluorescence Microscopy

Introduction to Fluorescent Proteins

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We’re happy to announce that we’ve updated our article on Single-Molecule Super-Resolution Imaging. This article now contains an in-depth discussion of sample preparation, including near-comprehensive tables listing known fluorescent probes and buffer systems for single molecule localization microscopies.

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    The resolving power of a microscope is the most important feature of the optical system.

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    The ratio of the speed of light in a vacuum to that in the imaging medium of a microscope.

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    Discussion of birefringence, Brewster's angle, and various forms of polarized light.

  • Introduction to Phase Contrast Microscopy

    A mechanism to translate variations in phase into corresponding changes in amplitude.


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    Spatial Resolution in Digital Imaging

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    Review the basic steps involved in acquiring a STORM super-resolution image.

  • Numerical Aperture and Image Resolution

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  • Eyepiece Reticle Calibration

    Explores calibration of various eyepiece reticles using a stage micrometer and demonstrates how the reticle can then be employed to determine linear specimen dimensions.


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Michael Davidson

Michael Davidson was a brilliant scientist, artist and photographer. The creator of MicroscopyU, Davidson also authored many scientific articles on the subject of photomicrography. His photomicrographs were published in more than a thousand national and international scientific journals, popular magazines and newspapers.  In addition, Davidson’s photomicrography won more than 40 awards in scientific and industrial photography competitions and has been exhibited at over 50 locations nationwide.

Learn more

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  • View Galleries of Nikon Small World Photomicrography Competition Winners
  • View Galleries of Nikon Small World in Motion Competition Winners
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Nikon Instruments Website Highlights

 

NIS.ai is a powerful new module for Nikon’s NIS-Elements imaging platform that enables you to:

• Virtually stain with Convert.ai
• Segment complex structures with Segment.ai
• Restore details to low-light images with Enhance.ai
• Remove shot noise from resonant confocal images with 登录国外网站的加速器

Learn more


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Nikon BioImaging Lab Symposium

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Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration

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